| Congresso Brasileiro de Microbiologia 2023 | Resumo: 827-2 | ||||
Resumo:Lipid synthesis by microorganisms using renewable resources has stimulated research focused on the development of economical and sustainable biotechnological alternatives. However, previous studies have demonstrated a limited number of yeast species identified for this purpose. This study aimed to evaluate lipid production by the unconventional yeast Torulaspora maleeae (CCMA 0039) under cultivation using sugarcane molasses and raw glycerol, and to characterize the fatty acid profile. Separate submerged cultures were conducted using 40g/L of each carbon source. Cultures with an inoculum of 107 cells/mL were incubated in an orbital shaker at 28°C with agitation at 150 rpm for 96 hours. Lipid extraction was performed using chloroform and methanol solvents, and the lipid content (g/L) was determined by calculating the lipid content relative to the dry weight of the collected biomass. The fatty acid profile was characterized using Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The T. maleeae strain showed lipid accumulation of 64.84% in raw glycerol medium and 31.38% in molasses medium. The fatty acid profile for cultures in both media was composed of palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), palmitoleic acid (C16:2), and linoleic acid (C18:2), which are highly valued by the food, pharmaceutical, and cosmetic industries due to their similarity to vegetable oil. However, for cultivation in raw glycerol, a higher total peak area of fatty acids and a higher relative percentage of linoleic acid were obtained. The yeast demonstrated efficiency in lipid accumulation using industrial by-products, although further studies related to cultivation conditions should be conducted to optimize productivity, aiming at applications in various industrial sectors. Palavras-chave: oleaginous yeast, industrial by- products, fatty acids Agência de fomento:CNPq, CAPES | |||||